Last Updated 11/1/07 MDR

MEDIUM

The recommended ratio of medium volume to surface area for monolayer cell cultures is 0.2-0.3 ml/cm2 *. The upper limit is set by gaseous diffusion through the liquid; the optimum ratio depends on the oxygen requirement of the cells. Cells with a high oxygen requirement grow better in shallow medium (~2mm), those with a low requirement grow better in deep medium (~5mm). Exceeding a medium depth of 5mm can hinder gas exchange and impair growth.

*96WP requires a ratio of 0.3-0.4 ml/cm2 to overcome surface tension's effect on cell distribution and growth in the wells

*Volumes used in tissue culture flasks are limited by the locations of the neck

TRYPSIN

PD: petri dish

WP: well plate

T: tissue culture flask

Dish Area (cm2)Medium Volume (ml)Trypsin-EDTA Volume (ml)
PD15176.735-505-7
PD1078.515-242-3
6WP9.61.9-2.90.40
12WP3.80.760-1.140.15
24WP1.80.360-0.5400.075
48WP0.75
96WP0.200.060-0.0800.01
T25255-61
T757515-202-3
T17517535-505-7